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KMID : 0061119820100000007
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Bulletin of the Clinical Research Institute
1982 Volume.10 No. 0 p.7 ~ p.7
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Autologous and Homologous bone Marrow Transplantation in Canine Model
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Kim, Hoon-Kyo
Kim, Choon-Choo/Kim, Dong-Jip
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Abstract
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The authors performed experimental autologous and homologous bone mar-row transplantation in canine model.
Seventy dogs were used for this study. Twelve dogs were used as control. -Thirty dogs were used as autologous model. Eighteen were conditioned with whole-body irradiation and 12 with intravenous cyclophosphamide. Twenty-eight dogs were used as homologous model. Allografts between mixed lymphocyte culture matched littermates following conditioned with whole-body irradiation were performed.
The results were as follows
1. Twelve control dogs died from, 6 to 12 days after irradiated or cyclophosphamide infusion.
2. In autologous series, the engraftment success rate was 27.8% in cyclophosphamide group and 66.8% in irradiation group. It was found that whole-body irradiation was superior to cyclophosphamide as conditioning regimen. In homologous series the engraftment success rate was 67.8%.
3. The numbers of nucleated bone marrow cells were closely related to the success of engraft and dog survival. An adequate number for engraftment in autologous transplantation was over 3 × 10^(8) cells/㎏, and optimal number for preventing graft failure and graft-versus-host reaction in allograft was inbetween 3 and 4 × 108 cells/㎏.
4. In autografts, white blood cell counts fell rapidly, reaching a nadir at 6 to 7 days. Normal levels were usually attained on the 12th day. Platelet counts diminished at the same rate as the white blood ` cell counts. ´ Normal levels were reached about 7 days later. Recovery of cellularity of bone marrow showed the same pattern as peripheral blood cells, but marrow cellularity did not always correlate well with peripheral blood counts.
5. In aliografts, the recovery of peripheral blood counts and of bone marrow cellularity was more gradual and slower than in autografts.
6. Marrow graft rejection occurred in 36.8% of engrafted dogs. Peripheral´ blood counts rapidly fell to aplastic level and bone marrow showed infiltration of p macrophages and mast cells with decreased cellularity.
7. In spite of methotrexate treatment, 52.6% of engrafted ;dogs succumbed to graft-versus-host reaction. In order to mitigate the rejection and graft-versushost reaction, complete dog leukocte-antigen (DL-A) identical donor must be matched using DL-A sera and mixed lymphocyte culture.
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KEYWORD
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